The international players' union wants FIFA to investigate why the Club World Cup match involving Western Sydney was played on a potentially dangerous rain-soaked field in Morocco.
FIFPro said letting the Wanderers-Cruz Azul quarter-final match continue in Rabat on Saturday "posed a serious threat to the health and safety of all players."
The Mexican club won 3-1 in extra time with players splashing through large puddles.
FIFPro official Tijs Tummers said "players as workers have a right to a safe work environment," adding FIFA must ensure "effective rules are in place to prevent a repetition."
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Referee Noumandiez Doue of Ivory Coast allowed the match to proceed, and Cruz Azul won.
FIFA then switched the semi-final match to Marrakech, citing "difficult pitch conditions."