Pain: Michael Clarke appeared to have strained his hamstring on day five in Adelaide. Photo: AP

Michael Clarke's international summer is again in doubt after suffering his second injury for the game in Adelaide on Saturday.

The captain limped off the field holding his right hamstring about 40 minutes after lunch on the fifth day. He was taken from the ground shortly afterwards for scans.

Clarke suffered the injury attempting to retrieve a ground ball at mid-wicket.

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It is the eighth time Clarke has injured his hamstrings.

Clarke had strained his left hamstring last month during a one-day international in Perth, raising doubts as to whether he would be fit for the start of the Test series against India.

The 33-year-old hurt his back on the first day of the Test while not out on 60 and was forced to retire hurt. He returned to the wicket the following morning after extensive treatment from physiotherapist Alex Kountouris on Tuesday night.

Clarke had already been in doubt to line up in Brisbane and the latest injury will end any hope he had of playing the second Test.

It will also have implications for his availability for the rest of the Test series and also raise questions over his participation in the World Cup.