The 19-time Grand Slam champion took umbrage with youngster after he touched some of his Nadal’s gear.
Nadal is renowned for his superstitious behaviour during games.
He has his drink bottles and racquets lined up in a meticulous manner for him to use when he changes ends.
“Something is happening on the side. Nadal (always) gives strict instructions to the ball boy not to touch something,” tennis legend Jim Courier said in commentary.
“Someone touched something,” American legend John McEnroe added.
“Mystery on Rod Laver Arena: Who touched what? A new series right after Married At First Sight.”
While his was occurring, a frustrated Kyrgios stood at the baseline waiting to serve.
It prompted fans to call for Nadal to be given a time-code violation.
Kyrgios and Nadal were involved in a war of words in the lead-up to the fourth round blockbuster, which is easily the most highly anticipated match so far this tournament